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May 22, 2007

PCR detetction of Salmonella food contamination not yet ready for prime time.

Analysis of: New Test May Allow For Rapid Detection Of Salmonella In Meat | www.medicalnewstoday.com
This analysis is solely the work of the author. It has not been edited or endorsed by GLG.
Analysis By:
Donald Thea
Professor of International Health, Boston University
Implications: PCR is an invaluable tool in the research setting but has only limited usefulness in a clinical context and, in my opinion, even more limited application for broad-based surveillance applications such as this. While PCR done well under laboratory conditions with samples spiked with known quantities of salmonella are able to be done with exquisite sensitivity (picking up all instances of salmonella) and specificity (not falsely picking salmonella when it isn't there), but getting these levels of test performance in an abbatoir or meat-packing facility will be challanging. PCR is notoriously susceptible to cross contamination and is very demanding of proper laboratory technique. Its ability to detect minutely small quantities (e.g. one bacteria or virus) of a pathogen make it prone to false positives. There are also many species of non-pathgenic Salmonella that might be detected (depending on the configuration of the primers used in the PCR) which may also result in false positives. 

Analysis: I don't see this as a metodology that the food industry will find applicable.

Other Analyses of the Same Source Article:
Cepheid's Value
June 12, 2007, Author: Ken Powell, President, Genesis Business Development
PCR analysis offers hope of pre-event detection of public health risks in food industry
May 29, 2007, Author: GLG Expert Contributor
PCR analysis offers hope of pre-event detection of public health risks in food industry
May 29, 2007, Author: GLG Expert Contributor
PCR analysis offers hope of pre-event detection of public health risks in food industry
May 28, 2007, Author: GLG Expert Contributor
PCR Used to Detect Bad Meat
May 23, 2007, Author: GLG Expert Contributor
Will it make our food supply safer?
May 22, 2007, Author: GLG Expert Contributor

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